实验动物科学 ›› 2023, Vol. 40 ›› Issue (1): 22-25.DOI: 10. 3969 / j. issn. 1006-6179. 2023. 01. 004

• 论著 • 上一篇    下一篇

制备神经源性膀胱大鼠模型的术前准备和术后护理

  

  1. ( 1. 陕西中医药大学,咸阳 712046) ( 2. 陕西中医药大学第二附属医院脑病二科,咸阳 712000) ( 3. 四川省南部县中医医院神经内科,南充 637300)
  • 收稿日期:2021-08-12 出版日期:2023-02-28 发布日期:2023-03-15
  • 通讯作者: 张润宁( 1973—) ,女, 硕士. 研究方向:脑卒中后康复. E-mail:xyzrn2000@ 163. com
  • 作者简介:刘 欣( 1995—) ,女,硕士. 研究方向:脊髓损伤后神经源性膀胱. E-mail:1010734147@ qq. com
  • 基金资助:
    陕西省中医药管理局科研项目( JCPT040) ;咸阳市科学技术研究发展计划 ( 2019k02-118) ;陕西中医药大学科学研究计划( 2017PY12) 

Pre-operative Preparation and Post-operative Care of a Rat Model of Neurogenic Bladder

  1. ( 1. Shaanxi University of Chinese Medicine, Xianyang 712046,China) ( 2. the Second Department of Encephalopathy, the Second Affiliated Hospital of Shaanxi University of Chinese Medicine, Xianyang 712000,China) ( 3. Department of Neurology, Nanbu County Hospital of Traditional Chinese Medicine, Nanchong 637300,China)
  • Received:2021-08-12 Online:2023-02-28 Published:2023-03-15

摘要: 目的 概述脊髓完全横断后神经源性膀胱大鼠模型术前准备中需要注意的事项及术后护理心得。 方法 购买雌性 SD 大鼠 35 只,横断 S3-S4 脊髓节段,建立逼尿肌无反射型神经源性膀胱大鼠模型,在术前、术后积极实施相关护理措施。 重点涉及手术前后的常规护理、术后尿道感染等并发症的防治及饲养管理等方面的内容。 结果 所有大鼠均在 S3-S4 脊髓节段横断脊髓,术后成活率为 82%(包含 1 只膀胱破裂和 2 只互相撕咬,3 只大鼠死于脓尿后败血症,术后 48 h 成活率为 100%) 。 结论 成熟的手术方法和熟练的手术操作对模型制作固然重要,精心的护理和完善的准备却是保障动物生命安全以及术后迅速恢复的关键环节。

关键词: 脊髓横断, 神经源性膀胱, 大鼠模型, 护理

Abstract: Objective To summarize the pre-operative preparation and post-operative nursing experience of neurogenic bladder after complete spinal cord transection in rats. Method Thirty-five female SD rats were purchased, and the rat model of detrusor non-reflex neurogenic bladder was established by transection of S3-S4 spinal cord. The nursing measures were taken before and after operation. It mainly involves the routine nursing before and after operation, the prevention and treatment of post-operative urethral infection and the management of feeding and so on. Result The spinal cord was transected at the S3-S4 segment in all rats, and the post-operative survival rate was 82%. Conclusion Although mature surgical method and skilled operation are important for the model making, careful care and perfect preparation are the key links to ensure the life safety and rapid recovery of animals after operation.

Key words: spinal cord transection, neurogenic bladder, rat model, nursing

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